Liquid Cytokines
Ready-to-use formulations – no reconstitution!
Simplify Your T-Cell Culture
Streamline your cell culture process with liquid-frozen formulations of animal-free RUO and GMP IL-2, IL-7, and IL-15. These ready-to-use cytokines eliminate reconstitution errors while providing the same potency and consistency as our lyophilized protein. Simply thaw and add to your cell culture!
Animal-Free RUO and GMP
ScaleReady utilizes Bio-Techne proteins produced in the only publicly known state-of-the-art certified animal-free facility, meaning you're working with truly animal-free devoid of animal contaminants and mammalian pathogens.
- Streamlined Process: Save time and materials - no reconstitution
- Increased Reproducibility: Eliminate errors with reconstitution steps
- Equivalent Bioactivity: Easily substitute for lyophilized proteins
- Stability*: Rely on full bioactivity for at least six months as supplied.
Liquid Animal Free RUO and GMP Proteins- TABLE
| GMP IL-2 | Lyophilized | Liquid |
|---|---|---|
| Formulation | Lyophilized from a 0.2 µm filtered solution in sodium acetate with Trehalose | 0.50 mg/ml, 0.2 µm filtered solution in sodium acetate |
| Stability | A minimum of 12 months when stored at ≤ -20°C as supplied | A minimum of 6 months when stored at ≤ -65°C as supplied |
| Catalog # | BT-002-GMP | BT-002-GMP/LQ |
| Pack Size | 50 µg, 1 mg | 50 µg, 1 mg |
| GMP IL-7 | Lyophilized | Liquid |
|---|---|---|
| Formulation | Lyophilized from a 0.2 µm filtered solution in PBS with Trehalose | 0.50 mg/ml, 0.2 µm filtered solution in PBS |
| Stability | A minimum of 12 months when stored at ≤ -20°C as supplied | A minimum of 6 months when stored at ≤ -65°C as supplied |
| Catalog # | BT-007-GMP | BT-007-GMP/LQ |
| Pack Size | 25 µg, 1 mg | 25 µg |
| GMP IL-15 | Lyophilized | Liquid |
|---|---|---|
| Formulation | Lyophilized from a 0.2 µm filtered solution in PBS with Trehalose | 0.50 mg/ml, 0.2 µm filtered solution in PBS |
| Stability | A minimum of 12 months when stored at ≤ -20°C as supplied | A minimum of 6 months when stored at ≤ -65°C as supplied |
| Catalog # | BT-015-GMP | BT-015-GMP/LQ |
| Pack Size | 25 µg, 1 mg | 25 µg |
Primary human T cell expansion was evaluated using both liquid format and lyophilized cytokines. Cells were expanded in G-Rex® 6M well plates using complete GMP Human T Cell Expansion Media supplemented with either 200 IU/mL GMP IL-2 or a combination of 10 ng/mL of both GMP IL-7 and GMP IL-15 in both liquid and lyophilized formats. After 9 days of expansion, cells were assessed for fold expansion and viability (left; data labels are mean % viability) as well as for memory phenotype using flow cytometry (right).
Direct comparisons of the liquid frozen (turquoise circles) and lyophilized (orange squares) formulations of GMP IL-2, IL-7, and IL-15. All 3 cytokines were tested in cell proliferation assays with no difference in bioactivity observed in any of the cytokines. IL-2 was tested on CTLL-2 mouse cytotoxic T cells, IL-7 was tested on PHA-activated human peripheral blood lymphocytes, and IL-15 was tested on MO7e human megakaryocytic leukemic cell.
How to order
Fill out the form below or contact us directly to place an order or consult with a ScaleReady expert.
Phone: 651-628-9259
Email: info@scaleready.com
Fax: 651-628-9507
Testimonials
Don’t cut corners on the cost of raw materials. Select high quality raw materials from the beginning.
- Michael Paglia
Chief Operating Officer
Elevate Bio, BaseCamp
Additional GMP Cytokine resources
Liquid Cytokines Brochure
Streamline your cell culture process with our new liquid frozen formulations of animal-free RUO and GMP IL-2, IL-7, and IL-15. These ready-to-use cytokines eliminate reconstitution errors while still providing the same potency and consistency as our lyophilized proteins. Simply thaw and add to your cell culture.
GMP Cytokines Optimization for Closed System T Cell Therapy Manufacturing
In this case study, a company focused on autologous T cell therapies targeting solid tumor cancers was progressing into clinical trials and needed to transition from research-grade to GMP-grade cytokines. Concurrently they wanted to improve
their methods of cytokine supplementation to reduce cytokine waste, improve dose accuracy across lots, and
reduce process risk by defining methods for closed-system cytokine addition. ScaleReady detailed a plan to help them achieve these goals.
Determining Optimal Seeding Density for Human T Cell Expansion in G-Rex
This scientific poster investigates the kinetics of human T cell expansion in small scale G-Rex6M bioreactors using ScaleReady reagents. Data show the affect of seeding density on fold expansion, total cell yield, and phenotypic phenotypic composition of expanded T cells, including total and memory CD4+ and CD8+ cells.
