Publications
Manufacture of GMP-grade Cytotoxic T Lymphocytes specific for LMP1 and LMP2 for Patients with EBV-associated Lymphoma
Outlines GMP manufacturing of LMP1/LMP2-specific CTLs for EBV-associated lymphoma. Describes using G-Rex devices for the second stimulation and expansion phases to support high-density cell growth. G-Rex simplifies the process and reduces labor compared to traditional 24-well plates while maintaining cell quality
Publications
Accelerated Production of Antigen-specific T Cells for Preclinical and Clinical Applications Using Gas-permeable Rapid Expansion Cultureware (G-Rex)
Evaluates G-Rex for expanding antigen-specific CTLs. G-Rex supports 3-20 fold higher cell output than 24-well plates by improving gas exchange and allowing deeper media. The device simplifies manufacturing by reducing interventions and supports high-density culture of CTLs and CAR-T cells without compromising function
Publications
Improved Islet Culture on Top of Gas-Permeable Membranes
Investigates culturing pancreatic islets on gas-permeable silicone rubber membranes. This method allows for higher surface densities (up to 4000 IE/cm²) and media depths compared to standard without hypoxic damage. G-Rex technology principles are tested here for islet survival and function
Publications
Development of large-scale expansion protocol for NKG2C-positive NK cells for treatment of glioblastoma
This study presents a protocol for large-scale expansion of NKG2C+ NK cells using PC-3 feeder cells. G-Rex 6M well plates were employed to achieve high expansion factors (median 358.5-fold) of functional NK cells for glioblastoma immunotherapy.