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The study analyses neoantigen-specific T cells in melanoma patients. Isolated neoTCRs were engineered into donor T cells, which were expanded in 24-well G-Rex plates. The expanded cells were used to validate TCR specificity and cytotoxicity against autologous tumor cell lines.

This study demonstrates that an oncolytic adenovirus encoding vIL-2 enhances NK cell cytotoxicity against ovarian cancer. Patient PBMCs used for humanized mouse models were expanded and rested using 6-well G-Rex plates with TILs media.

The paper presents a low-cost, point-of-care CAR-T manufacturing process. It uses the Sepax C-Pro for cell processing and G-Rex devices for cell expansion. The study demonstrates that this simplified workflow produces highly functional CAR-T cells, comparable to standard methods, supporting decentralized production.

This study evaluates anti-CD20 pre-treatment for enhancing SIV-specific CAR-T cell therapy in macaques. CAR-T cells were manufactured using G-Rex 6-well plates for expansion. While anti-CD20 treatment increased initial CAR-T expansion, the cells failed to persist long-term, and viral loads were not significantly affected.

The study develops an activated T cell (ATC) therapy targeting glioblastoma cancer stem cells. It compares antigen sources, finding synthetic peptide pools most effective. T cells were co-cultured with antigen-loaded dendritic cells in G-Rex 100 containers for activation and rapid proliferation over 12 days.

This review discusses the biomanufacturing of NK cell therapies, covering cell sources, expansion methods, and process control. It identifies G-Rex as a commonly used culture system for clinical-scale NK cell production due to their gas-permeable membranes, facilitating efficient cell expansion.

This review explores the role of small extracellular vesicles (sEVs) derived from various immune cells. It highlights a protocol using G-Rex to produce and isolate large amounts of human NK cell-derived sEVs, demonstrating their potential for clinical application in cancer therapy.

High-throughput screening identified a small molecule, GSK2622391, that enhances lentiviral transduction efficiency in T cells. The compound improves vector copy number without negatively impacting cell viability or function. G-Rex plates were used for T cell expansion during small-scale validation experiments.

This study characterizes humoral and T cell responses to the BNT162b2 vaccine. It establishes a rapid 11-day ex-vivo expansion protocol for spike-specific T cells using G-Rex 10 devices, demonstrating significant fold expansion and functional enhancement for potential adoptive immunotherapy.

This review covers various bioreactor systems for red blood cell production. It specifically references the use of G-Rex for the expansion of peripheral blood mononuclear cells (PB MNCs) in its summary of 3D scaffolds and bioreactors.

The study shows that overexpression of an engineered PGC-1α improves the metabolic fitness and anti-tumor efficacy of CAR-T cells. During in vitro culture, cells were transferred to G-Rex 6M Well Plates for rapid expansion.

This research explores the mechanisms of immune evasion related to CD58 loss and PD-L1 upregulation. Patient-derived TILs and CRISPR-engineered T cells used in the experiments were expanded using G-Rex 10 and G-Rex 100 bottles.