Knowledge HQ

This paper investigates how tuning T-cell stimulation dose using APC-mimetic scaffolds affects CAR-T phenotype and function across healthy and patient samples. 6-well G-Rex plates were used for the expansion of CAR-T cells, enabling the generation of diverse cell products for analysis.

The authors analyzed lentiviral vector integration sites in SCID-X1 patients and CD19-CAR T cells to identify safety and efficacy signatures. G-Rex 6-well and 100M plates were utilized for culturing transduced T cells, facilitating the generation of cell products for integrome analysis.

This research combines affinity-tuned EpCAM/ICAM-1 CARs with NFAT-inducible IL-12 to enhance antitumor activity in solid tumors while minimizing toxicity. G-Rex 6M well plates were employed to expand the CAR-T cell products after lentiviral transduction.

This study develops a protocol for manufacturing base-edited CAR-T cells using circular RNA (circRNA) to reduce costs and improve stability compared to linear mRNA. Large-scale production of PD-1 deficient CAR-T cells was achieved using 100M and six-well G-Rex vessels, demonstrating scalability.

This phase I trial evaluates locoregional B7-H3 CAR-T cells for DIPG. The clinical CAR-T cell products were manufactured using G-Rex 100MCS vessels, incorporating a methotrexate selection step to enrich for modified cells.

The study reports on the IMA101 clinical trial. Endogenous multi-target T cells were primed and expanded using G-Rex 100MCS and G-Rex 100M vessels in a rapid expansion protocol, enabling the generation of large cell numbers for patient infusion.

The paper describes a manufacturing strategy for Aspergillus-specific T cells (ATCs). A short-term expansion (STE) protocol using G-Rex devices allowed for the generation of clinically relevant numbers of functional ATCs within 12 days.

This study develops Dox-inducible lentiviral vectors for CAR-T cells. G-Rex plates were used for the production of monoclonal antibodies from hybridomas and for expanding transduced primary T cells in the absence of Dox prior to in vivo experiments.

The study evaluates an oncolytic adenovirus expressing vIL-2 to boost TIL therapy for ovarian cancer. Autologous TILs were expanded from patient tumor fragments using six-well G-Rex plates as part of the 'young' TILs expansion protocol.

This paper describes a pooled screening strategy for CAR domain combinations using DNA barcoding. G-Rex plates were utilized for co-culturing transduced T cells with tumor cells during repeated antigen stimulation assays to identify CAR constructs with superior expansion and proliferation.

The study investigates T cell responses to SARS-CoV-2 and endemic coronaviruses. It describes a protocol to generate universal multi-coronavirus specific T cells (MCVSTs) by expanding PBMCs in G-Rex gas-permeable containers, yielding robust cell numbers for potential adoptive immunotherapy.

The study isolates neutralizing human mAbs against Sosuga virus from a survivor. Hybridoma cell lines secreting these mAbs were expanded in 6-well G-Rex plates to produce sufficient antibodies for purification and subsequent functional characterization.