Knowledge HQ

Describes generating CTL lines targeting multiple tumor-associated antigens (SSX2, MAGEA4, Survivin, etc.) for lymphoma. Uses optimized cytokine cocktails (IL-7, IL-12, IL-15, IL-6/IL-27) for activation. While not explicitly detailed in the excerpts, G-Rex is standard for this group's expansion protocols

Describes using the PiggyBac transposon system to modify EBV-CTLs to express HER2-CARs. The G-Rex device (G-Rex10 and G-Rex100) was used to expand the gene-modified CTLs, supporting long-term survival and stable transgene expression. G-Rex allowed efficient expansion of clinical-grade cells with minimal manipulation

Reviews optimization of antigen-specific T cell manufacturing. Highlights the G-Rex device as a revolutionary tool that increases cell yields by 3-20 fold while reducing manipulation. G-Rex allows unrestricted media depth due to gas exchange from the bottom, linearly scalable from research to GMP production

Outlines GMP manufacturing of LMP1/LMP2-specific CTLs for EBV-associated lymphoma. Describes using G-Rex devices for the second stimulation and expansion phases to support high-density cell growth. G-Rex simplifies the process and reduces labor compared to traditional 24-well plates while maintaining cell quality

Evaluates G-Rex for expanding antigen-specific CTLs. G-Rex supports 3-20 fold higher cell output than 24-well plates by improving gas exchange and allowing deeper media. The device simplifies manufacturing by reducing interventions and supports high-density culture of CTLs and CAR-T cells without compromising function

Investigates culturing pancreatic islets on gas-permeable silicone rubber membranes. This method allows for higher surface densities (up to 4000 IE/cm²) and media depths compared to standard without hypoxic damage. G-Rex technology principles are tested here for islet survival and function

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Ignacio Nunez discusses the importance of collaboration and partnerships, such as that between ScaleReady and Cellular Origins, and the impact of these on drug development and patient accessibility.

The best technology is often the simplest. Quiet, elegant, brilliant. A revolution in T-Cell culture technology. The G-Rex Bioreactor.

Discover a game-changing approach to T cell expansion. This application note showcases how the G-Rex bioreactor and Bio-Techne reagents deliver hands-free, zero-touchpoint cell growth. Learn how to streamline your manufacturing, boost efficiency, and maintain cell health—all with a simple fill-and-forget workflow.