TcBuster™ non-viral gene delivery system for cell and gene therapy
Gene editing without the viral vector baggage.
How does TcBuster gene delivery work?
TcBuster integrates your gene of interest (GOI) into host cell DNA using the highly efficient Red Flour Beetle transposase and specifically-designed transposons. Transposon gene editing systems have the safest insertion profile of any commercial gene delivery platform, with limited off-target effects and stable, reliable gene expression.
Insert large CAR payloads and maintain high efficiency.
TcBuster is advancing TCR and CAR-T engineering by accommodating insertion of large and multi-gene constructs. This allows you to create innovative, highly targeted, and controlled gene-modified immune cell therapies. Using optimized protocols, TcBuster transposition efficiency even outperforms lentivirus transduction.
CAR and TCR gene transfer without the viral vector baggage.
TcBuster addresses the cost, scalability, and integration challenges of retroviral and lentiviral vectors, as demonstrated in the table below. Manufacturing of TcBuster plasmids is inherently faster and less variable than retro and lentiviral production creating a more secure supply chain to support your gene edited immune cell therapies.
Scalable gene editing to support cell therapy manufacturing.
TcBuster gene edited immune cells are compatible with cell expansion in closed-system G-Rex bioreactors. Optimized transposition and expansion protocols will support research and therapeutic scales.
TcBuster gene editing service options
Additional TcBuster resources
TcBuster Non-viral Genetic Engineering Flyer
- How TcBuster works
- Comparison to viral tranduction
- Data on transposition efficiency and scalability