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This study evaluated the safety of intra-arterial TIL delivery in rabbits. The human TILs used for infusion were expanded from glioblastoma tumors using G-Rex to obtain sufficient cell numbers for the experiment.

The authors developed GM-CSF Receptor (CD116) CAR T cells using the piggyBac transposon system. The transgenic CAR T cells were expanded in G-Rex 10 devices to generate effector cells for testing against JMML cells.

The study reports that culturing juvenile porcine islets in Wilson Wolf gas-permeable (G-Rex) allows them to aggregate into functional 'pancreatites'. These flasks were also used for shipping the islets, maintaining viability and function.

Describes a GMP-compliant method for expanding NK cells using K562 feeder cells in G-Rex 10 and G-Rex 100. The method yields large numbers of functional NK cells in 10 days without feeding, and supports efficient retroviral transduction.

The study demonstrates successful expansion of tumor-reactive TILs from pancreatic cancer. The Rapid Expansion Protocol (REP) was performed using GREX10 to stimulate and expand the TILs with anti-CD3 and feeder cells.

This paper details the GMP manufacture of Cytovir ADV. The process utilizes a closed G-Rex 10 cell culture device for a 10-day expansion of adenovirus-specific T cells, requiring minimal intervention and achieving high purity and potency.

This study shows efficient mRNA electroporation of NK cells. Clinical-grade NK cells were expanded ex vivo using G-Rex prior to electroporation, allowing for the generation of large numbers of highly cytotoxic cells.

This paper investigates the use of G-Rex for TIL rapid expansion. Results show G-Rex rescues poor TIL growth, preserves clonal diversity, and yields cells with a more oxidative metabolic phenotype (higher mitochondrial capacity) compared to traditional flasks/bags.

The study validates a xeno-free serum replacement for T cell expansion. It demonstrates that combining OpTmizer medium with CTS Immune Cell SR in G-Rex culture vessels promotes optimal expansion of antigen-specific T cells compared to standard flasks.

The authors developed a method for early transduction of trivirus-specific T cells. The clinical SOP utilized G-Rex100 for the second stimulation phase to rapidly expand transduced VSTs to clinically relevant numbers.

The study found that IL-12 conditioning increases IL-7Rα, crucial for T cell persistence. For clinical translation, TCR-transduced human T cells were expanded using a rapid expansion protocol in G-Rex 100 to generate large numbers of cells for analysis.

This document provides instructions for Lonza's Fresh Human Pancreatic Islets. It highlights that the islets are shipped in Wilson Wolf G-Rex® gas permeable cell culture devices (G-Rex10 or G-Rex100) to maintain viability during transport and culture.