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Generated MUC1-specific CAR T cells co-expressing an inverted cytokine receptor (4/7ICR) for breast cancer. Co-culture experiments to monitor anti-tumor activity were conducted in 6-well G-Rex devices using a 3D algimatrix bioscaffold.

Investigated methods to overcome fratricide in NKG2D-CAR T cell production. The manufacturing process included an expansion phase in G-Rex containers, which was part of the upscaling strategy to produce sufficient cell yields.

Evaluated Lym-1 CAR T cells against B-cell lymphoma. Primary T cells were transduced and expanded in 24-well G-Rex plates. The cells showed potent anti-tumor effects in vitro and in vivo against Raji xenografts.

Study on 4-1BB agonist enhancing CD8+ TIL growth from pancreatic ductal adenocarcinoma (PDAC). The Rapid Expansion Protocol (REP) was performed in G-Rex 10 devices, enabling the exponential growth of sorted CD8+ and bulk TIL populations for functional assays.

Describes a culture method using GM-CSF, TLR agonists, and IL-7 to expand antigen-specific T-cells from PBMC. The study confirmed the culture method could be scaled up using G-Rex 100M vessels, showing effectiveness comparable to 24-well plates for patient treatment.

Describes Iovance's Gen 2 manufacturing process for LN-144 (TILs), an abbreviated 22-day closed process yielding cryopreserved product. The Gen 2 process uses a single closed bioreactor to improve scalability and throughput compared to the Gen 1 method.

Investigates mesothelin as an immunotherapeutic target in glioblastoma. Mesothelin-specific T-cell responses were detected in TILs. G-Rex were utilized for the further expansion of TILs isolated from glioma tumor tissue to achieve necessary cell numbers.

Developed a rapid method to produce multivirus-specific T cells from cord blood using overlapping PepMixes. G-Rex culture devices maintained or improved antigen recognition and overcame limitations in expanding sufficient cells for clinical application, allowing large-scale production.

Describes a clinical-scale protocol to generate autologous BK virus-specific T cell lines from kidney transplant recipients. G-Rex10 vessels were used for all T cell cultures, allowing for rapid expansion and generation of antigen-specific T cell lines suitable for adoptive immunotherapy.

Describes a method for large-scale isolation of extracellular vesicles (EVs) from activated NK cells. The G-Rex cell culture device allowed for the expansion of 1-3 billion aNK cells per 100 cm2, increasing EV production while reducing cost and complexity. Isolated EVs showed cytotoxicity against cancer cells.

A single-cell methodology integrating cellular phenotyping and dynamic cytokine secretion was developed using nanowell arrays. G-Rex 100M were used for the rapid expansion protocol (REP) of tumor-infiltrating lymphocytes (TILs) to achieve desired cell numbers for experiments.

This study showed that collecting PBMCs after exercise increases the yield of VSTs. The VSTs were expanded in G-Rex10 for 8 days, demonstrating that exercise is a simple adjuvant for improving VST manufacture.