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The authors developed a scalable, serum-free process for lentiviral vector production using suspension-adapted SJ293TS cells. The vectors efficiently transduced human T cells and CD34+ cells. G-Rex plates were employed to expand the transduced T cells for assessing chimeric antigen receptor expression and titer.

This study compares in-house produced CD19 CAR-T cells for ALL and NHL. Cells from ALL patients expanded better. Production utilized G-Rex100 , enabling rapid generation of therapeutic doses. The study highlights phenotypic differences based on the patient's disease.

The authors describe a GMP-compliant method to rapidly expand fungus-specific T cells using CD137 selection and short-term culture. G-Rex cell culture plates were used for the in vitro expansion of the enriched CD137+ population. The resulting T cells demonstrated potent antifungal activity against Aspergillus.

This study compares monocyte-derived dendritic cell (Mo-DC) production in fluorinated ethylene propylene bags versus polystyrene plates. Mo-DCs generated in bags showed comparable phenotype and ability to activate T cells. G-Rex were utilized for the T cell co-culture steps to assess activation potency.

Evaluated TIL ACT for ovarian cancer. TILs were expanded using a 'TIL 3.0' method combining IL-2 with agonistic 4-1BB and anti-CD3 antibodies in G-Rex 10. This method significantly increased CD8+ TIL yield and success rates compared to IL-2 alone, producing tumor-reactive T cells in 2-3 weeks.

Production of a third-party CMV-specific T cell (CMVST) bank. Cells were expanded using G-Rex 5 and G-Rex 100M. The bank successfully provided matched lines for 96.6% of screened patients with 100% response rate in treated patients.

Study improving Vg9Vd2 T cell responses using NKG2D RNA CARs. Cells were expanded using K562 aAPCs in G-Rex vessels before electroporation. CAR-modified cells showed enhanced cytotoxicity against solid tumors in vitro and in vivo.

Study identifying E3L as a key antigen for protective T-cell immunity against vaccinia virus. Virus-specific T cells (VSTs) were generated, referencing previous protocols using G-Rex cultureware.

Case report of a pediatric patient with relapsed Ewing sarcoma treated with intratumoral injections of NK-92 cells. NK-92 cells were expanded in GMP conditions using the G-Rex culture system. Local tumor regression was observed.

Development of 'NKF' feeder cells (OCI-AML3 with mbIL-21) for NK expansion. The system allows large-scale expansion (>10,000 fold) and was validated in G-Rex. Expanded NK cells showed high cytotoxicity and metabolic activation.

Demonstrated multiplex base editing (PDCD1, TRAC, B2M) in T cells to create allogeneic CAR-T cells. Edited cells were expanded in G-Rex vessels. The method reduced double-strand breaks and translocations compared to Cas9 nuclease.

Study on extracellular vesicles (EVs) derived from NK cells. NK cells were propagated and activated using K562-mbIL21 aAPCs in G-Rex culture devices. NK-EVs contained cytotoxic proteins and induced cell death in cancer cells via multiple mechanisms.