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This review summarizes the current landscape of CAR T-cell therapy for mantle cell lymphoma. It discusses manufacturing processes, noting that selection, activation, transduction, and expansion steps are often performed in systems such as G-Rex bottles.

The study demonstrates that CoStAR, a chimeric costimulatory receptor, enhances the effector function of T cells and TILs. Transgene-expressing cells and TILs were expanded using G-Rex plates, including G-Rex 6M, as part of the rapid expansion protocol.

This study investigates the role of CDK6 and PTPs in regulating T cell activity and immunotherapy resistance. CD19-CAR T cells used in the study were expanded in G-Rex 10 and G-Rex 100 to generate sufficient cell numbers.

The authors demonstrate a method to generate CAR-NK cells using mRNA-LNP transfection. The NK cells used in the study were cultured and expanded in G-Rex 6-well and 24-well plates.

The authors describe a manufacturing process for expanding virus-specific CD8+ T cells from vaccinated macaques. The final co-culture of T cells, PHA blasts, and feeder cells was rapidly expanded in G-Rex 100 for one week.

This paper presents a novel macroencapsulation device for islet transplantation to treat type 1 diabetes. While the study focuses on device engineering, it references previous work utilizing G-Rex technology for cell culture applications.

This phase 1 trial used non-viral CRISPR-Cas9 editing to replace endogenous TCRs with personalized neoantigen-specific TCRs in T cells. Clinical-grade manufacturing of the neoTCR T cell products included activation and expansion steps performed in G-Rex 100M CS

The paper describes a system (CISC) that allows engineered T cells and Tregs to be selectively expanded using rapamycin. Clinical-scale manufacturing of the engineered cells was demonstrated using G-Rex 100M CS vessels.

This study optimized CRISPR/Cas9 editing of expanded primary human NK cells. Ablation of SYK enhanced ADCC and cytokine production. Post-nucleofection, the edited NK cells were transferred to G-Rex 24-well plates for culture and recovery.

This study presents Chimeric Engulfment Receptor (CER) T cells that target phosphatidylserine to clear tumors and present antigens. The manufacturing process for these modified T cells involved transferring transduced cells to 6-well G-Rex dishes for expansion.

The researchers developed a monoclonal antibody against Syrian hamster PD-L1 to enable immunotherapy modeling. In the study, hamster tumor-infiltrating lymphocytes (TILs) were isolated and cultured in six-well G-Rex plates.

The study characterizes the expansion and differentiation of cord blood-derived gamma-delta T cells. A modified rapid expansion protocol (REP) utilizing G-Rex was employed to achieve scalable expansion of different gamma-delta T cell subtypes.