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This paper describes a virus-free CAR-T manufacturing process using the Quantum pBac system and Quantum Booster supplement. Use of G-Rex 24-well and 1L vessels allowed for the generation of clinically relevant numbers of high-quality CAR-TSCM cells in 10 days.

This study identifies that GZMB leakage causes apoptosis in cryopreserved NK cells and that IL-15/IL-18 pretreatment mitigates this. NK cells were isolated and expanded for 15-20 days in G-Rex 6-well plates using irradiated K562 feeder cells.

The researchers designed a split-signaling bispecific CAR to target AML cells while sparing HSCs. CAR T cells were expanded using G-REX plates to generate sufficient cell numbers for in vivo xenograft experiments comparing bispecific and monospecific CARs.

The study presents a logic-gated CAR-T strategy to safely target HER2 by sparing normal HLA-A*02+ cells. G-Rex 24 or 6-well plates were utilized for the expansion of CAR-T cells after lentiviral transduction, supporting the generation of cells for in vitro and in vivo assays.

This paper demonstrates that the CD8α hinge/transmembrane domain improves GUCY2C CAR-T cell affinity and antitumor efficacy compared to CD28 domains. CAR-T cells were expanded ex vivo for 12 days using G-Rex 6-well plates prior to cryopreservation.

The authors developed leucine zipper-based receptors (ZipRs) to mimic cytokine signaling in CAR-T cells, enhancing their function. Autonomous cell outgrowth assays were conducted by seeding ZipR-transduced T cells in G-REX 6-well plates to ensure safety.

Describes DARIC33, a CD33-targeted CAR-T controlled by rapamycin to mitigate AML toxicity. Clinical-scale T cell products were manufactured using G-Rex vessels, demonstrating process feasibility and producing cells with high engraftment potential.

This study demonstrates that G-Rex devices allow for a 20-fold increase in islet seeding density compared to T-flasks without compromising viability or function. Modeling and experiments confirm that the gas-permeable membrane prevents anoxia in high-density cultures.

The authors found that sequential stimulation with mbIL21 then mbIL15 feeder cells optimizes NK cell yield and function. Purified NK cells were co-cultured with irradiated K562 feeders in G-Rex 6-well plates, facilitating robust expansion for functional assessment.

This protocol details a medium-throughput method to clone and screen TCRs against various antigens using combinatorial dye staining. G-Rex 24-well plates are recommended for expanding TCR-transduced T cells after Dynabead removal to ensure sufficient cell numbers for screening.

This clinical trial assesses TIL therapy in refractory solid tumors. While no objective responses were seen, disease control was observed. The Rapid Expansion Protocol (REP) utilized G-Rex 10 and 100M to generate the large numbers of TILs required for patient infusion.

The study describes PC-CARs targeting the neuroblastoma oncoprotein PHOX2B presented on HLA. These CARs kill tumor cells across multiple HLA allotypes. Primary human T cells were activated and expanded in G-Rex system vessels (Wilson Wolf) during the retroviral and lentiviral transduction processes.