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SOP for initiating CTLs. Describes initiating cells in '24-well plate or GRex40 '. Details seeding 1x10^7 cells in 5mL for G-Rex40 and culturing for 9 days for initial stimulation

SOP for transducing LCLs with adenovirus vectors. G-Rex is not explicitly referenced in this document, though it is part of a larger G-Rex workflow for CTL production

SOP for freezing CTLs. Mentions harvesting CTLs from 'wells or GRex '. Provides a protocol for ensuring cell quality and viability during cryopreservation

SOP for generating LCLs. Mentions expansion of LCLs in ' (G40 and G500)' (G-Rex) if large numbers are required. Describes seeding densities and media volumes for G-Rex40 (30 mL) and G-Rex500 (100-200 mL)

Details the GMP manufacturing process for trivirus-specific CTLs (CMV, EBV, Adv). The protocol allows for stimulation and expansion in either 24-well plates or G-Rex devices (specifically G-Rex40 and G-Rex500). G-Rex is noted for supporting high-density cell growth and simplifying the process

Describes a static culture protocol for expanding NK cells using K562-mb15-41BBL feeder cells in G-Rex devices. The G-Rex system yielded higher fold expansion (>100-fold) and total cell numbers compared to gas-permeable bags, with no feeding required during the 8-10 day culture period

Details a simplified TIL production method using G-Rex. G-Rex10 is used for initial culture from tumor fragments/digests, and G-Rex100 for rapid expansion (REP). This method reduces media use by 3-4 times and requires fewer vessels and labor compared to traditional 24-well plates and bags

Describes a rapid method (10 days) to generate multivirus-specific CTLs using pepmixes and IL4/IL7. The method uses the G-Rex10 device for stimulation and expansion, allowing the production of large numbers (1x10^8) of virus-specific T cells from a small starting number of PBMCs with minimal manipulation

Compares the WAVE to static gas-permeable bags for TIL and PBL expansion. While total cell numbers were comparable, the WAVE produced a higher percentage of CD4+ cells and a less activated phenotype. The study references G-Rex (Vera 2010) as an alternative static option

Describes generating CTL lines targeting multiple tumor-associated antigens (SSX2, MAGEA4, Survivin, etc.) for lymphoma. Uses optimized cytokine cocktails (IL-7, IL-12, IL-15, IL-6/IL-27) for activation. While not explicitly detailed in the excerpts, G-Rex is standard for this group's expansion protocols

Describes using the PiggyBac transposon system to modify EBV-CTLs to express HER2-CARs. The G-Rex device (G-Rex10 and G-Rex100) was used to expand the gene-modified CTLs, supporting long-term survival and stable transgene expression. G-Rex allowed efficient expansion of clinical-grade cells with minimal manipulation

Reviews optimization of antigen-specific T cell manufacturing. Highlights the G-Rex device as a revolutionary tool that increases cell yields by 3-20 fold while reducing manipulation. G-Rex allows unrestricted media depth due to gas exchange from the bottom, linearly scalable from research to GMP production