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Describes a protocol for expanding nTregs using G-Rex10 and G-Rex100 devices. The method yielded >10^9 Tregs in 21 days with high purity and suppressive function. G-Rex promoted robust expansion (over 600-fold) compared to 24-well plates (5-6 fold) and maintained telomere length

Clinical trial results for TIL therapy in melanoma. Mentions using 'gas-permeable bags or GRex ' for the Rapid Expansion Procedure (REP). Notes that G-Rex allow large-scale expansion to treatment levels (10^10 - 10^11 cells)

SOP for expanding CTLs. Detailed instructions for using G-Rex40 and G-Rex500. Describes transfer of cells to G-Rex, adding media and IL-2, feeding schedules (removing half media), and splitting cells if density is high

SOP for initiating CTLs. Describes initiating cells in '24-well plate or GRex40 '. Details seeding 1x10^7 cells in 5mL for G-Rex40 and culturing for 9 days for initial stimulation

SOP for transducing LCLs with adenovirus vectors. G-Rex is not explicitly referenced in this document, though it is part of a larger G-Rex workflow for CTL production

SOP for freezing CTLs. Mentions harvesting CTLs from 'wells or GRex '. Provides a protocol for ensuring cell quality and viability during cryopreservation

SOP for generating LCLs. Mentions expansion of LCLs in ' (G40 and G500)' (G-Rex) if large numbers are required. Describes seeding densities and media volumes for G-Rex40 (30 mL) and G-Rex500 (100-200 mL)

Details the GMP manufacturing process for trivirus-specific CTLs (CMV, EBV, Adv). The protocol allows for stimulation and expansion in either 24-well plates or G-Rex devices (specifically G-Rex40 and G-Rex500). G-Rex is noted for supporting high-density cell growth and simplifying the process

Describes a static culture protocol for expanding NK cells using K562-mb15-41BBL feeder cells in G-Rex devices. The G-Rex system yielded higher fold expansion (>100-fold) and total cell numbers compared to gas-permeable bags, with no feeding required during the 8-10 day culture period

Details a simplified TIL production method using G-Rex. G-Rex10 is used for initial culture from tumor fragments/digests, and G-Rex100 for rapid expansion (REP). This method reduces media use by 3-4 times and requires fewer vessels and labor compared to traditional 24-well plates and bags

Describes a rapid method (10 days) to generate multivirus-specific CTLs using pepmixes and IL4/IL7. The method uses the G-Rex10 device for stimulation and expansion, allowing the production of large numbers (1x10^8) of virus-specific T cells from a small starting number of PBMCs with minimal manipulation

Compares the WAVE to static gas-permeable bags for TIL and PBL expansion. While total cell numbers were comparable, the WAVE produced a higher percentage of CD4+ cells and a less activated phenotype. The study references G-Rex (Vera 2010) as an alternative static option