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The authors developed a method for early transduction of trivirus-specific T cells. The clinical SOP utilized G-Rex100 for the second stimulation phase to rapidly expand transduced VSTs to clinically relevant numbers.

The study validates a xeno-free serum replacement for T cell expansion. It demonstrates that combining OpTmizer medium with CTS Immune Cell SR in G-Rex culture vessels promotes optimal expansion of antigen-specific T cells compared to standard flasks.

This document provides instructions for Lonza's Fresh Human Pancreatic Islets. It highlights that the islets are shipped in Wilson Wolf G-Rex® gas permeable cell culture devices (G-Rex10 or G-Rex100) to maintain viability during transport and culture.

The study found that IL-12 conditioning increases IL-7Rα, crucial for T cell persistence. For clinical translation, TCR-transduced human T cells were expanded using a rapid expansion protocol in G-Rex 100 to generate large numbers of cells for analysis.

This paper establishes a clinical-compliant protocol to generate MiHA-specific T-cell lines. The priming phase utilized G-Rex10 to maximize cell expansion and DC-T cell interactions starting from limited PBMCs, followed by a rapid expansion protocol.

The study demonstrates that AML exosomes suppress hematopoiesis by altering stromal cells and progenitors. To model physiological hypoxia, AML cells were cultured in G-Rex in a low-oxygen chamber, which increased exosome release compared to normoxia.

This study describes a BiTE derived from a TCR-mimic antibody against WT1. It induced T-cell cytotoxicity against WT1+ tumors and epitope spreading. Transduced EBV-specific T lymphocytes used in in vivo tracking studies were cultured in G-Rex.

The authors developed Go-CART, a six-chambered device to assess CAR T cell function without animals. The device features a gas-permeable base, allowing prolonged T cell-tumor interaction (>2 weeks) without medium replenishment, similar to G-Rex technology (co-author John Wilson is from Wilson Wolf).

This study demonstrates that the immune system can mount a mutation-specific Th1 response against epithelial cancer. Adoptive transfer of enriched mutation-specific CD4+ TILs led to tumor regression. The provided excerpts do not explicitly mention the use of G-Rex devices.

This study generated CD19-specific CAR T cells using the piggyBac transposon system and expanded them in G-Rex 10 devices under serum-free conditions. This protocol produced clinically relevant numbers of CAR T cells that were effective against TKI-resistant Ph+ ALL cells.

This study demonstrates that CAR-redirected EBV-CTLs engineered to express IL-7Ra can be selectively expanded by IL-7 in the presence of Tregs. G-Rex were used to generate the expanded Tregs used in the suppression assays. This strategy enhances antitumor activity.

This abstract reports the clinical use of rapidly generated pentavalent virus-specific T cells expanded in G-Rex devices. The single-stimulation G-Rex protocol produced sufficient cells in 10 days to safely and effectively treat drug-refractory EBV, CMV, Adv, BK, and HHV6 infections in HSCT patients.