Knowledge HQ

Investigated methods to overcome fratricide in NKG2D-CAR T cell production. The manufacturing process included an expansion phase in G-Rex containers, which was part of the upscaling strategy to produce sufficient cell yields.

Generated MUC1-specific CAR T cells co-expressing an inverted cytokine receptor (4/7ICR) for breast cancer. Co-culture experiments to monitor anti-tumor activity were conducted in 6-well G-Rex devices using a 3D algimatrix bioscaffold.

Evaluated Lym-1 CAR T cells against B-cell lymphoma. Primary T cells were transduced and expanded in 24-well G-Rex plates. The cells showed potent anti-tumor effects in vitro and in vivo against Raji xenografts.

Study on 4-1BB agonist enhancing CD8+ TIL growth from pancreatic ductal adenocarcinoma (PDAC). The Rapid Expansion Protocol (REP) was performed in G-Rex 10 devices, enabling the exponential growth of sorted CD8+ and bulk TIL populations for functional assays.

Describes Iovance's Gen 2 manufacturing process for LN-144 (TILs), an abbreviated 22-day closed process yielding cryopreserved product. The Gen 2 process uses a single closed bioreactor to improve scalability and throughput compared to the Gen 1 method.

Describes a culture method using GM-CSF, TLR agonists, and IL-7 to expand antigen-specific T-cells from PBMC. The study confirmed the culture method could be scaled up using G-Rex 100M vessels, showing effectiveness comparable to 24-well plates for patient treatment.

Investigates mesothelin as an immunotherapeutic target in glioblastoma. Mesothelin-specific T-cell responses were detected in TILs. G-Rex were utilized for the further expansion of TILs isolated from glioma tumor tissue to achieve necessary cell numbers.

Describes a clinical-scale protocol to generate autologous BK virus-specific T cell lines from kidney transplant recipients. G-Rex10 vessels were used for all T cell cultures, allowing for rapid expansion and generation of antigen-specific T cell lines suitable for adoptive immunotherapy.

Developed a rapid method to produce multivirus-specific T cells from cord blood using overlapping PepMixes. G-Rex culture devices maintained or improved antigen recognition and overcame limitations in expanding sufficient cells for clinical application, allowing large-scale production.

A single-cell methodology integrating cellular phenotyping and dynamic cytokine secretion was developed using nanowell arrays. G-Rex 100M were used for the rapid expansion protocol (REP) of tumor-infiltrating lymphocytes (TILs) to achieve desired cell numbers for experiments.

Describes a method for large-scale isolation of extracellular vesicles (EVs) from activated NK cells. The G-Rex cell culture device allowed for the expansion of 1-3 billion aNK cells per 100 cm2, increasing EV production while reducing cost and complexity. Isolated EVs showed cytotoxicity against cancer cells.

This study showed that collecting PBMCs after exercise increases the yield of VSTs. The VSTs were expanded in G-Rex10 for 8 days, demonstrating that exercise is a simple adjuvant for improving VST manufacture.