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The authors developed leucine zipper-based receptors (ZipRs) to mimic cytokine signaling in CAR-T cells, enhancing their function. Autonomous cell outgrowth assays were conducted by seeding ZipR-transduced T cells in G-REX 6-well plates to ensure safety.

This paper demonstrates that the CD8α hinge/transmembrane domain improves GUCY2C CAR-T cell affinity and antitumor efficacy compared to CD28 domains. CAR-T cells were expanded ex vivo for 12 days using G-Rex 6-well plates prior to cryopreservation.

The study presents a logic-gated CAR-T strategy to safely target HER2 by sparing normal HLA-A*02+ cells. G-Rex 24 or 6-well plates were utilized for the expansion of CAR-T cells after lentiviral transduction, supporting the generation of cells for in vitro and in vivo assays.

This study demonstrates that G-Rex devices allow for a 20-fold increase in islet seeding density compared to T-flasks without compromising viability or function. Modeling and experiments confirm that the gas-permeable membrane prevents anoxia in high-density cultures.

Describes DARIC33, a CD33-targeted CAR-T controlled by rapamycin to mitigate AML toxicity. Clinical-scale T cell products were manufactured using G-Rex vessels, demonstrating process feasibility and producing cells with high engraftment potential.

This clinical trial assesses TIL therapy in refractory solid tumors. While no objective responses were seen, disease control was observed. The Rapid Expansion Protocol (REP) utilized G-Rex 10 and 100M to generate the large numbers of TILs required for patient infusion.

This protocol details a medium-throughput method to clone and screen TCRs against various antigens using combinatorial dye staining. G-Rex 24-well plates are recommended for expanding TCR-transduced T cells after Dynabead removal to ensure sufficient cell numbers for screening.

The authors found that sequential stimulation with mbIL21 then mbIL15 feeder cells optimizes NK cell yield and function. Purified NK cells were co-cultured with irradiated K562 feeders in G-Rex 6-well plates, facilitating robust expansion for functional assessment.

This paper investigates how tuning T-cell stimulation dose using APC-mimetic scaffolds affects CAR-T phenotype and function across healthy and patient samples. 6-well G-Rex plates were used for the expansion of CAR-T cells, enabling the generation of diverse cell products for analysis.

The study describes PC-CARs targeting the neuroblastoma oncoprotein PHOX2B presented on HLA. These CARs kill tumor cells across multiple HLA allotypes. Primary human T cells were activated and expanded in G-Rex system vessels (Wilson Wolf) during the retroviral and lentiviral transduction processes.

This research combines affinity-tuned EpCAM/ICAM-1 CARs with NFAT-inducible IL-12 to enhance antitumor activity in solid tumors while minimizing toxicity. G-Rex 6M well plates were employed to expand the CAR-T cell products after lentiviral transduction.

The authors analyzed lentiviral vector integration sites in SCID-X1 patients and CD19-CAR T cells to identify safety and efficacy signatures. G-Rex 6-well and 100M plates were utilized for culturing transduced T cells, facilitating the generation of cell products for integrome analysis.