Learn from Dr. Rooney at the “Ask me Anything” session during this conference. She will be covering T-cell biology, manufacturing, potency testing and specificity.Dr. Rooney is a translational immunologist with longstanding expertise in the development, genetic engineering, and clinical translation of virus-specific T cells (VSTs) and other adoptive cellular immunotherapies for cancer and virus-associated diseases. Her work has centered on defining how intrinsic properties of the T-cell chassis, including antigen specificity, differentiation state, tissue trafficking, and persistence, govern the efficacy and safety of engineered T-cell therapies. Over the course of her career, she has helped establish antigen-specific T cells as versatile therapeutic platforms for lymphoma, solid tumors, and post-transplant complications.A major focus of her research has been the development of Epstein–Barr virus-specific T cells (EBVSTs) as a cellular platform with advantages over conventional polyclonally activated T cells, including enhanced persistence, tissue homing, and antigen-driven survival. Her laboratory has contributed to their generation, characterization, and clinical application in both autologous and allogeneic settings, demonstrating their safety, long-term persistence, and therapeutic activity. Building on this foundation, her team is now engineering EBVSTs with chimeric antigen receptors (CARs) to improve tumor targeting while preserving the favorable in vivo biology of memory-derived, antigen-specific T cells.A central theme of her work is how to enhance the potency of engineered T cells without increasing immune effector cell–associated toxicities. To address this, her laboratory has developed strategies incorporating constitutive cytokine signaling, safety switches, and combinatorial targeting approaches. These studies support the hypothesis that combining the intrinsic biology of EBVSTs with engineered receptors can yield cellular therapies with improved efficacy and a wider therapeutic window.In parallel with her research program, she leads translational and educational efforts in cell and gene therapy at Baylor College of Medicine, including the Translational Research Laboratories and the Immune Effector Cell Manufacturing Group of their GMP facility. These roles span discovery, translational development, GMP manufacturing, and clinical implementation, enabling the efficient advancement of engineered T-cell therapies from concept to clinic.